Results
| PMID | 11821289 |
| Gene Name | TNF |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 130 |
| Population details | 130 (56 with endometriosis, 8 with idiopathic infertility, 27 controls (tubal ligation), 39 were excluded due to bloody PF) |
| Sex | Female |
| Associated genes | IL-1beta, IL-6, IL-8, IL-12, IL-13, TNF-alpha |
| Other associated phenotypes |
Endometriosis |
|
Hum Reprod. 2002 Feb;17(2):426-31. Bedaiwy, M A| Falcone, T| Sharma, R K| Goldberg, J M| Attaran, M| Nelson, D R| Agarwal, A Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. BACKGROUND: The objective of this prospective controlled trial was to investigate the ability of a group of serum and peritoneal fluid (PF) markers to predict, non-surgically, endometriosis. METHODS AND RESULTS: Serum and PF samples were obtained from 130 women while undergoing laparoscopy for pain, infertility, tubal ligation or sterilization reversal. Concentrations of six cytokines [interleukin (IL)-1beta, IL-6, IL-8, IL-12, IL-13 and tumour necrosis factor (TNF)-alpha] were measured in serum and PF, and reactive oxygen species (ROS) in PF, and levels were compared among women who were allocated to groups according to their post-surgical diagnosis. Fifty-six patients were diagnosed with endometriosis, eight with idiopathic infertility, 27 underwent tubal ligation or reanastomosis (control group) and 39 were excluded due to bloody PF. Only serum IL-6 and PF TNF-alpha could be used to discriminate between patients with and without endometriosis with a high degree of sensitivity and specificity (P < 0.001). A threshold of 15 pg/ml PF TNF-alpha provided 100% sensitivity and 89% specificity (positive likelihood ratio of 9.1 and negative likelihood ratio of 0). A threshold of 2 pg/ml for serum IL-6 provided a sensitivity of 90% and specificity of 67% (positive likelihood ratio of 2.7 and negative likelihood ratio of 0.14). CONCLUSIONS: By measuring serum IL-6 and PF TNF-alpha, it was possible to discriminate between patients with endometriosis and those without. Before these markers can be used as a non-surgical diagnostic tool, these data should be verified in a larger study. Mesh Terms: Adult| Ascitic Fluid/*metabolism| Biomarkers/*blood| Cytokines/blood/metabolism| Diagnosis, Differential| Endometriosis/blood/diagnosis/*metabolism| Female| Forecasting| Humans| Interleukin-6/blood| Likelihood Functions| Prospective Studies| R |
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